roche hiv nucleic acid detection kit instructions Related introduction

(Re)search the easy way - Roche Applied Science

Products are for life science research only. Not for use in diagnostic procedures unless otherwise indicated. This website contains information on products which is targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country.Improving HIV-2 Detection by a Combination of Serological roche hiv nucleic acid detection kit instructionsFor HIV-1 RNA purification, the High Pure viral nucleic acid isolation kit was used with 500 l of plasma, according to the manufacturer's instructions (Roche Diagnostics, Mannheim, Germany). The amplification and detection were performed in an automated Cobas TaqMan 48 analyzer by using the hydrolysis probe technology with the Cobas TaqMan roche hiv nucleic acid detection kit instructions

MagNA Pure LC Total Nucleic Acid Kit - High Performance

Feb 16, 2018 · The kit is function tested by isolation of total viral nucleic acids (DNA and RNA) from human reference material using the Total NA HP 200 purification protocol. The purified total viral nucleic acids are then detected by quantitative, real-time PCR and RT-PCR using virus-specific assays established for the LightCycler ® System.ACCELERATED EMERGENCY USE AUTHORIZATION (EUA) Instructions are included in the kit to direct the home users on roche hiv nucleic acid detection kit instructions Volume Kit on the Roche MagNA Pure-96 (MP6), and Applied Biosystems roche hiv nucleic acid detection kit instructions contain nucleic acid for testing. 4) A negative roche hiv nucleic acid detection kit instructionsAssessment of eight nucleic acid amplification roche hiv nucleic acid detection kit instructionsApr 22, 2019 · Nucleic acid amplification technologies (NAATs) are high-performance tools for rapidly and accurately detecting infectious agents. They are widely used in high-income countries to diagnose disease and improve patient care. The complexities associated with test methods, reagents, equipment, quality control and assurance require dedicated laboratories with trained staff, which can exclude their roche hiv nucleic acid detection kit instructions

CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT

The CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel is a molecular in vitro diagnostic test that aids in the detection and diagnosis 2019-nCoV and is based on widely used nucleic acid amplificationClinical Validation of a Novel Diagnostic HIV-2 Total roche hiv nucleic acid detection kit instructionsBackground. The 2014 CDC 4 th generation HIV screening algorithm includes an orthogonal immunoassay to confirm and discriminate HIV-1 and HIV-2 antibodies. Additional nucleic acid testing (NAT) is recommended to resolve indeterminate or undifferentiated HIV seroreactivity. HIV-2 NAT requires a second-line assay to detect HIV-2 total nucleic acid (TNA) in patients blood cells, as a Clinical Validation of a Novel Diagnostic HIV-2 Total roche hiv nucleic acid detection kit instructionsBackground. The 2014 CDC 4 th generation HIV screening algorithm includes an orthogonal immunoassay to confirm and discriminate HIV-1 and HIV-2 antibodies. Additional nucleic acid testing (NAT) is recommended to resolve indeterminate or undifferentiated HIV seroreactivity. HIV-2 NAT requires a second-line assay to detect HIV-2 total nucleic acid (TNA) in patients blood cells, as a

Cobas® TaqMan® HIV-1 Test v2 - Roche Diagnostics

Intended use The COBAS ® TaqMan ® HIV-1 Test, version 2.0 (v2.0) For Use With The High Pure System is an in vitro nucleic acid amplification test for the quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human plasma, using the High Pure System Viral Nucleic Acid Kit for manual specimen preparation and the COBAS ® TaqMan ® 48 Analyzer for automated amplification and detection.Complete List of DSA for Infectious Agents and HIV roche hiv nucleic acid detection kit instructionsHIV-1 Nucleic Acid Assays - Human Immunodeficiency Virus Type 1 Anti-HIV Specimen Collection Devices, Testing Services, and Home Test Kits Anti-HIV-2 Assays - Human Immunodeficiency Virus Complete List of DSA for Infectious Agents and HIV roche hiv nucleic acid detection kit instructionsHIV-1 Nucleic Acid Assays - Human Immunodeficiency Virus Type 1 Anti-HIV Specimen Collection Devices, Testing Services, and Home Test Kits Anti-HIV-2 Assays - Human Immunodeficiency Virus

DIG Application Manual 3rd - lifescience.roche roche hiv nucleic acid detection kit instructions

However, preparing nucleic acid probes with a stable nonradioactive label removes the major obstacles which hinder the general application of in situ hybridization. Further-more, it opens new opportunities for combining different labels in one experiment. The many sensitive antibody detection systems available for such probes further enhances theDIG Luminescent Detection Kit - Sigma-AldrichDetection Kit y Version 08 Content version: September 2018 Chemiluminescent detection of digoxigenin labeled nucleic acids by enzyme-immunoassay Cat. No. 11 363 514 910 Kit for the detection of 50 blots of 100 cm2 Store the kit at 15 to 25°C Please note: The antibody (vial 3) and the substrate CSPD (vial 5), once opened, should be stored at +2 roche hiv nucleic acid detection kit instructionsDiagnostic detection of 2019-nCoV by real-time RT-PCRDetection of these relatively distant members of the SARS-related CoV clade suggests that all Asian viruses are likely to be detected. Specificity testing 1. Chemical stability To exclude non-specific reactivity of oligonucleotides among each other, both assays were tested 40 times in parallel with water and no other nucleic acid except the roche hiv nucleic acid detection kit instructions

Donor Screening - Nucleic Acid Testing (NAT)

Donor Screening - Nucleic Acid Testing (NAT) Over 60 million blood and plasma donations are tested on Roche NAT systems today. 1 From launching the first Nucleic Acid Testing assays for blood screening, Roche has consistently remained in the forefront in keeping the blood supply safe through active surveillance of emerging infections and roche hiv nucleic acid detection kit instructionsHigh Pure RNA Isolation Kit - Roche Applied ScienceNucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. For RNA isolation, the binding conditions can be optimized to ensure immobilization of all the RNA.High Pure Viral Nucleic Acid Kit - Roche Applied ScienceThe High Pure Viral Nucleic Acid Kit is intended for general laboratory use and efficiently purifies viral nucleic acids from serum, plasma, or whole blood using sample volumes of up to 200 L. When using whole blood as starting material, total nucleic acids are isolated, including viral nucleic acids.

High Pure Viral Nucleic Acid Kit | Roche | Bioz

For HIV-positive samples, DNAs were typically extracted from 200,000 PBMCs (High Pure viral nucleic acid kit; Roche Diagnostics, Mannheim, Germany), and screening for the HIV-1 subtype was done by a multiregion hybridization assay (MHAacd) ( ) on an ABI 7700 sequence detection system (Applied Biosystems, Darmstadt, Germany). .. Plasma viral roche hiv nucleic acid detection kit instructionsHigh Pure Viral Nucleic Acid Large Volume KitFigure 1: Crossing Points of a series dilution of HAV particles in human citrate plasma after isolation with the High Pure Viral Nucleic Acid Large Volume Kit and subsequent analysis on the LightCycler ® 2.0 Instrument. 1 ml citrated plasma was spiked with decreasing amounts of HAV viral particles (1 × 10 5 to 1 × 10 2 particles). Isolation was performed according to the Instructions for Use.Human immunodeficiency virus (HIV) - Roche DiagnosticsHuman immunodeficiency virus (HIV) roche hiv nucleic acid detection kit instructions As the leading provider of HIV early infant diagnosis and viral load testing, Roche has a social responsibility to provide care where access is limited, especially in countries hardest hit by the disease. roche hiv nucleic acid detection kit instructions nucleic acid amplification technology for blood and plasma. More .

Improving HIV-2 Detection by a Combination of Serological roche hiv nucleic acid detection kit instructions

For HIV-1 RNA purification, the High Pure viral nucleic acid isolation kit was used with 500 l of plasma, according to the manufacturer's instructions (Roche Diagnostics, Mannheim, Germany). The amplification and detection were performed in an automated Cobas TaqMan 48 analyzer by using the hydrolysis probe technology with the Cobas TaqMan roche hiv nucleic acid detection kit instructionsIn Vitro Tests to Detect Nucliec Acid Sequences of HIV 1 & 2As of October 1999, there were 27 licensed kits and 5 premarket approvals (PMAs) for detection of antibodies to HIV-1 or HIV-2 in blood, saliva, or urine, which included: 19 Enzyme-Linked roche hiv nucleic acid detection kit instructionsMagNA Pure Compact Nucleic Acid Isolation Kit I - Large For details, see - Instructions for Use To isolate genomic DNA or total nucleic acid from smaller-volume samples (100 to 400 l), choose the MagNA Pure Compact Nucleic Acid Isolation Kit I Product Citations. Binnicker, MJ et al. (2007). Detection of Coccidioides Species in Clinical Specimens by Real-Time PCR. J. Clin. Microbiol. 45: 173-178

MagNA Pure LC Total Nucleic Acid Kit - High Performance

Feb 16, 2018 · The kit is function tested by isolation of total viral nucleic acids (DNA and RNA) from human reference material using the Total NA HP 200 purification protocol. The purified total viral nucleic acids are then detected by quantitative, real-time PCR and RT-PCR using virus-specific assays established for the LightCycler ® System.MycoTOOL Mycoplasma Detection Amplification KitThe MycoTOOL Mycoplasma Detection Amplification Kit is an in vitro nucleic acid amplification test optimized for the detection of mycoplasma in CHO cell culture according to the Guideline for Mycoplasma NAT described in chapter 2.6.7 of the European Pharmacopeia with respect to specificity, sensitivity/detection limit, and robustness. In roche hiv nucleic acid detection kit instructionsQC Sample Preparation Kit - custombiotech.roche roche hiv nucleic acid detection kit instructionsDetection of mycoplasma contamination. Using a PCR block cycler, refer to the method sheet (instructions for use) of the MycoTOOL Mycoplasma Detection Amplification Kit. - for testing of CHO or SP2/0 cells with 5 x 10 6 cells/ml (standard protocol) - for testing of CHO or SP2/0 samples up to 1 x 10 8 cells/ml (protocol for high cell density)

Table 3. Nucleic Acid Detection Based Tests | CDC

List may not include all available test kits approved by the FDA. These specimen types are specified in product package inserts cleared by the U.S. Food and Drug Administration (FDA) Test Time is inclusive of actual test time and is exclusive of transport, handling, laboratory run Table 3. Nucleic Acid Detection Based Tests | CDCList may not include all available test kits approved by the FDA. These specimen types are specified in product package inserts cleared by the U.S. Food and Drug Administration (FDA) Test Time is inclusive of actual test time and is exclusive of transport, handling, laboratory run The Stability of HIV-1 Nucleic Acid in Whole Blood and roche hiv nucleic acid detection kit instructions3.1. Specimen Handling and Shipping for Proficiency Testing: Prior to collecting whole blood units for use in creating panels for qualitative HIV-1 nucleic acid proficiency testing, samples from donors enrolled in the VQA Program [Jackson et al., 1993] were screened to confirm their HIV-1 serostatus, plasma viral load and CD4 T-cell counts.HIV-1 RNA testing (plasma) was done during a screening roche hiv nucleic acid detection kit instructions

The stability of HIV-1 nucleic acid in whole blood and roche hiv nucleic acid detection kit instructions

HIV-1 nucleic acids are stable in refrigerated whole blood for 10 days. Whole blood can be frozen or processed into alternative specimen types that improve HIV-1 detection over DBS. Modified Roche TaqMan HIV-1 RNA assay can be used to quantify total HIV-1 nucleic acids in whole blood.The stability of HIV-1 nucleic acid in whole blood and roche hiv nucleic acid detection kit instructionsBACKGROUND: Commercially-available kits for HIV-1 detection include instructions for detecting HIV-1 in plasma and DBS, but don't support other specimen types. OBJECTIVES: Show quantitative stability of HIV-1 total nucleic acid (TNA) in blood and improved HIV-1 detection in alternative specimen types.The stability of HIV-1 nucleic acid in whole blood and roche hiv nucleic acid detection kit instructionsNov 01, 2018 · Data generated in this study show that HIV-1 total nucleic acid (TNA) can be 320 times higher in 0.1 mL BLD compared to the nucleic acid eluted from a 75 uL spot. Donor characteristics (specifically viral load and HIV-1 TNA in BLD) contributed to the variability in the detection of HIV-1 nucleic acid in DBS samples.

cobas HCV

cobas® HCV 08111952001-01EN Doc. Rev. 1.0 4 Intended use cobas® HCV is an in vitro nucleic acid amplification test for both the detection and quantitation of hepatitis C virus (HCV) RNA, in human EDTA plasma or serum, of HCV-infected individuals. Samples containing HCV genotype 1 to 6cobas HCVcobas® HCV 08111952001-01EN Doc. Rev. 1.0 4 Intended use cobas® HCV is an in vitro nucleic acid amplification test for both the detection and quantitation of hepatitis C virus (HCV) RNA, in human EDTA plasma or serum, of HCV-infected individuals. Samples containing HCV genotype 1 to 6cobas® HIV-1 Test - Roche DiagnosticsJul 14, 2016 · US-IVD. The cobas ® HIV-1 is an in vitro nucleic acid amplification test for the quantitation of human immunodeficiency virus type 1 (HIV-1) in EDTA plasma of HIV-1-infected individuals using the automated cobas ® 6800/8800 Systems for specimen processing, amplification and detection. The test can quantitate HIV-1 RNA over the range of 20-10,000,000 copies/mL (33 to 1.67 x 10 7

cobas® HIV-1 Test - Roche Diagnostics

The cobas ® HIV-1 is an in vitro nucleic acid amplification test for the quantitation of human immunodeficiency virus type 1 (HIV-1) in EDTA plasma of HIV-1-infected individuals using the automated cobas ® 6800/8800 Systems for specimen processing, amplification and detection.cobas® HIV-1/HIV-2 Qualitative Test - Roche DiagnosticsIntended use The cobas ® HIV-1/HIV-2 Qualitative nucleic acid test for use on the cobas ® 6800/8800 Systems is an in vitro nucleic acid amplification test for the qualitative detection and differentiation of human immunodeficiency virus (HIV) type 1 (HIV-1) and type 2 (HIV-2) in human serum, plasma, and dried blood spots (DBS).. The test is intended to be used as an aid in diagnosis of HIV-1 roche hiv nucleic acid detection kit instructions

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